Publications of J. Rajan Prabu

Horn-Ghetko, D.; Hopf, L. V. M.; Tripathi-Giesgen, I.; Du, J.; Kostrhon, S.; Tung Vu, D.; Beier, V.; Steigenberger, B.; Prabu, J. R.; Stier, L. et al.; Bruss, E. M.; Mann, M.; Xiong, Y.; Schulman, B. A.: Noncanonical assembly, neddylation and chimeric cullin-RING/RBR ubiquitylation by the 1.8 MDa CUL9 E3 ligase complex. Nature Structural & Molecular Biology 31 (7), pp. 1083 - 1094 (2024)

Li, J.; Purser, N.; Liwocha, J.; Scott, D. C.; Byers, H. A.; Steigenberger, B.; Hill, S.; Tripathi-Giesgen, I.; Hinkle, T.; Hansen, F. M. et al.; Prabu, J. R.; Radhakrishnan, S. K.; Kirkpatrick, D. S.; Reichermeier, K. M.; Schulman, B. A.; Kleiger, G.: Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting. Molecular Cell 84 (7), pp. 1304 - 1320 (2024)

Liwocha, J.; Li, J.; Purser, N.; Rattanasopa, C.; Maiwald, S.; Krist, D. T.; Scott, D. C.; Steigenberger, B.; Prabu, J. R.; Schulman, B. A. et al.; Kleiger, G.: Mechanism of millisecond Lys48-linked poly-ubiquitin chain formation by cullin-RING ligases. Nature Structural & Molecular Biology 31 (2), pp. 378 - 389 (2024)

Hehl, L. A.; Horn-Ghetko, D.; Prabu, J. R.; Vollrath, R.; Tung Vu, D.; Pérez Berrocal, D. A.; Mulder, M. P. C.; van der Heden van Noort, G. J.; Schulman, B. A.: Structural snapshots along K48-linked ubiquitin chain formation by the HECT E3 UBR5. Nature Chemical Biology 20, pp. 190 - 200 (2024)

Chrustowicz, J.; Sherpa, D.; Li, J.; Langlois, C. R.; Papadopoulou, E. C.; Tung Vu, D.; Hehl, L. A.; Karayel, Ö.; Beier, V.; Gronau, S. v. et al.; Müller, J.; Prabu, J. R.; Mann, M.; Kleiger, G.; Alpi, A. F.; Schulman, B. A.: Multisite phosphorylation dictates selective E2-E3 pairing as revealed by Ubc8/UBE2H-GID/CTLH assemblies. Molecular Cell 84 (2), pp. 293 - 308 (2024)

Wallace, I.; Baek, K.; Prabu, J. R.; Vollrath, R.; von Gronau, S.; Schulman, B. A.; Swatek, K. N.: Insights into the ISG15 transfer cascade by the UBE1L activating enzyme. Nature Communications 14 (1), 7970 (2023)

Chrustowicz, J.; Sherpa, D.; Teyra, J.; Loke, M. S.; Popowicz, G. M.; Basquin, J.; Sattler, M.; Prabu, J. R.; Sidhu, S. S.; Schulman, B.: Multifaceted N-Degron Recognition and Ubiquitylation by GID/CTLH E3 Ligases. Journal of Molecular Biology 434 (2), 167347 (2022)

Ferguson, C. J.; Urso, O.; Bodrug, T.; Gassaway, B. M.; Watson, E. R.; Prabu, J. R.; Lara-Gonzalez, P.; Martinez-Chacin, R. C.; Wu, D. Y.; Brigatti, K. W. et al.; Puffenberger, E. G.; Taylor, C. M.; Haas-Givler, B.; Jinks, R. N.; Strauss, K. A.; Desai, A.; Gabel, H. W.; Gygi, S. P.; Schulman, B. A.; Brown, N. G.; Bonni, A.: APC7 mediates ubiquitin signaling in constitutive heterochromatin in the developing mammalian brain. Molecular Cell 82 (1), pp. 90 - 105 (2022)

Horn-Ghetko, D.; Krist, D. T.; Prabu, J. R.; Baek, K.; Mulder, M. P. C.; Kluegel, M.; Scott, D. C.; Ovaa, H.; Kleiger, G.; Schulman, B.: Ubiquitin ligation to F-box protein targets by SCF–RBR E3–E3 super-assembly. Nature 590 (7847), pp. 671 - 676 (2021)

Kostrhon, S.; Prabu, J. R.; Baek, K.; Horn-Ghetko, D.; Gronau, S. v.; Klügel, M.; Basquin, J.; Alpi, A. F.; Schulman, B. A.: CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific NEDD8 activation. Nature Chemical Biology 17 (10), pp. 1075 - 1083 (2021)

Sherpa, D.; Chrustowicz, J.; Qiao, S.; Langlois, C. R.; Hehl, L. A.; Gottemukkala, K. V.; Hansen, F. M.; Karayel, O.; von Gronau, S.; Prabu, J. R. et al.; Mann, M.; Alpi, A. F.; Schulman, B. A.: GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme. Molecular Cell 81 (11), pp. 2445 - 2459.e13 (2021)

Baek, K.; Krist, D. T.; Prabu, J. R.; Hill, S.; Klügel, M.; Neumaier, L.-M.; von Gronau, S.; Kleiger, G.; Schulman, B. A.: NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligation assembly. Nature 578, pp. 461 - 466 (2020)

Miller-Vedam, L. E.; Bräuning, B.; Popova, K. D.; Oakdale, N. T. S.; Bonnar, J. L.; Prabu, J. R.; Boydston, E. A.; Sevillano, N.; Shurtleff, M. J.; Stroud, R. M. et al.; Craik, C. S.; Schulman, B. A.; Frost, A.; Weissman, J. S.: Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. eLife 9, e62611 (2020)

Oh, E.; Mark, K. G.; Mocciaro, A.; Watson, E. R.; Prabu, J. R.; Cha, D. D.; Kampmann, M.; Gamarra, N.; Zhou, C. Y.; Rape, M.: Gene expression and cell identity controlled by anaphase-promoting complex. Nature 579, pp. 136 - 140 (2020)

Qiao, S.; Langlois, C. R.; Chrustowicz, J.; Sherpa, D.; Karayel, O.; Hansen, F. M.; Beier, V.; von Gronau, S.; Bollschweiler, D.; Schäfer, T. et al.; Alpi, A. F.; Mann, M.; Prabu, J. R.; Schulman, B.: Interconversion between Anticipatory and Active GID E3 Ubiquitin Ligase Conformations via Metabolically Driven Substrate Receptor Assembly. MOLECULAR CELL 77 (1), pp. 150 - 163.e9 (2020)

Schuller, S. K.; Schuller, J. M.; Prabu, J. R.; Baumgärtner, M.; Bonneau, F.; Basquin, J.; Conti, E.: Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex. eLife 9, e61467 (2020)

Watson, E. R.; Grace, C. R. R.; Zhang, W.; Miller, D. J.; Davidson, I. F.; Prabu, J. R.; Yu, S.; Bolhuis, D. L.; Kulko, E. T.; Vollrath, R. et al.; Haselbach, D.; Stark, H.; Peters, J.-M.; Brown, N. G.; Sidhu, S. S.; Schulman, B. A.: Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site. Proceedings of the National Academy of Sciences of the United States of America 116 (35), pp. 17280 - 17289 (2019)

Ozgur, S.; Buchwald, G.; Falk, S.; Chakrabarti, S.; Prabu, J. R.; Conti, E.: The conformational plasticity of eukaryotic RNA-dependent ATPases. The FEBS Journal 282 (5), pp. 850 - 863 (2015)

Prabu, J. R.; Müller, M.; Thomae, A. W.; Schüssler, S.; Bonneau, F.; Becker, P. B.; Conti, E.: Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling. MOLECULAR CELL 60 (3), pp. 487 - 499 (2015)

Schmalen, I.; Reischl, S.; Wallach, T.; Klemz, R.; Grudziecki, A.; Prabu, J. R.; Benda, C.; Kramer, A.; Wolf, E.: Interaction of Circadian Clock Proteins CRY1 and PER2 Is Modulated by Zinc Binding and Disulfide Bond Formation. CELL 157 (5), pp. 1203 - 1215 (2014)