Publications
2024
Alexander Kögel, Achim Keidel, Matina-Jasemi Loukeri, Christopher C. Kuhn, Lukas M. Langer, Ingmar B. Schäfer, and Elena Conti, "Structural basis of mRNA decay by the human exosome–ribosome supercomplex," Nature 635, 237-242 (2024).
Lukas M. Langer, Katharina Kurscheidt, Jérôme Basquin, Fabien Bonneau, Iuliia Iermak, Claire Basquin, and Elena Conti, "UPF1 helicase orchestrates mutually exclusive interactions with the SMG6 endonuclease and UPF2," Nucleic Acids Research 52 (10), 6036-6048 (2024).
Sofia Todesca, Felix Sandmeir, Achim Keidel, and Elena Conti, "Molecular basis of human polyA polymerase recruitment by mPSF," RNA: A Publication of the RNA Society 30 (7), 795-806 (2024).
2023
Patrik Polák, William Garland, Om Rathore, Manfred Schmid, Anna Salerno-Kochan, Lis Jakobsen, Maria Gockert, Piotr Gerlach, Toomas Silla, Jens S. Andersen, Elena Conti, and Torben Heick Jensen, "Dual agonistic and antagonistic roles of ZC3H18 provide for co-activation of distinct nuclear RNA decay pathways," Cell Reports 42 (11), 113325 (2023).
Achim Keidel, Alexander Kögel, Peter Reichelt, Eva Kowalinski, Ingmar B. Schäfer, and Elena Conti, "Concerted structural rearrangements enable RNA channeling into the cytoplasmic Ski238-Ski7-exosome assembly," Molecular Cell 83 (22), 4093-4105 (2023).
Fabien Bonneau, Jérôme Basquin, Barbara Steigenberger, Tillmann Schäfer, Ingmar B. Schäfer, and Elena Conti, "Nuclear mRNPs are compact particles packaged with a network of proteins promoting RNA–RNA interactions," Genes & Development 37 (11-12), 505-517 (2023).
Jérôme O. Rouvière, Anna Salerno-Kochan, Søren Lykke-Andersen, William Garland, Yuhui Dou, Om Rathore, Ewa Šmidová Molska, Guifen Wu, Manfred Schmid, Andrii Bugai, Lis Jakobsen, Kristina Žumer, Patrick Cramer, Jens S. Andersen, Elena Conti, and Torben Heick Jensen, "ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts," Molecular Cell 83 (13), 2240-2257 (2023).
Ji Huang, Xiangyang Liu, Yadong Sun, Zhuang Li, Min-Han Lin, Keith Hamilton, Corey R. Mandel, Felix Sandmeir, Elena Conti, Paul H. Oyala, and Liang Tong, "An examination of the metal ion content in the active sites of human endonucleases CPSF73 and INTS11," Journal of Biological Chemistry 299 (4), 103047 (2023).
2022
Fabienne Mauxion, Jerome Basquin, Sevim Ozgur, Marion Rame, Jana Albrecht, Ingmar Schäfer, Bertrand Séraphin, and Elena Conti, "The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions," Cell Reports 42 (1), 111902 (2023).
Johanna B. Brüggenthies, Alessandra Fiore, Marion Russier, Christina Bitsina, Julian Brötzmann, Susanne Kordes, Sascha Menninger, Alexander Wolf, Elena Conti, Jan E. Eickhoff, and Peter J. Murray, "A cell-based chemical-genetic screen for amino acid stress response inhibitors reveals torins reverse stress kinase GCN2 signaling," Journal of Biological Chemistry 298 (12), 102629 (2022).
Piotr Gerlach, William Garland, Mahesh Lingaraju, Anna Salerno-Kochan, Fabien Bonneau, Jerome Basquin, Torben Heick Jensen, and Elena Conti, "Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome," Molecular Cell 82 (13), 2505-2518 (2022).
Moritz Schmidt, Florian Kluge, Felix Sandmeir, Uwe Kühn, Peter Schäfer, Christian Tüting, Christian Ihling, Elena Conti, and Elmar Wahle, "Reconstitution of 3′ end processing of mammalian pre-mRNA reveals a central role of RBBP6," Genes & Development 36 (3-4), 195-209 (2022).
Alexander Kögel, Achim Keidel, Fabien Bonneau, Ingmar Schaefer, and Elena Conti, "The human SKI complex regulates channeling of ribosome-bound RNA to the exosome via an intrinsic gatekeeping mechanism," Molecular Cell 82 (4), 756-769 (2022).
2021
Lukas M. Langer, Fabien Bonneau, Yair Gat, and Elena Conti, "Cryo-EM reconstructions of inhibitor-bound SMG1 kinase reveal an autoinhibitory state dependent on SMG8," eLife 10, e72353 (2021).
2020
Sandra K. Schuller, Jan M. Schuller, J. Rajan Prabu, Marc Baumgärtner, Fabien Bonneau, Jerome Basquin, and Elena Conti, "Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex," eLife 9, e61467 (2020).
Mahesh Lingaraju, Jan M. Schuller, Sebastian Falk, Piotr Gerlach, Fabien Bonneau, Jerome Basquin, Christian Benda, and Elena Conti, "To Process or to Decay: A Mechanistic View of the Nuclear RNA Exosome," Cold Spring Harbor Symposia on Quantitative Biology 84, 155-163 (2019).
Lukas M. Langer, Yair Gat, Fabien Bonneau, and Elena Conti, "Structure of substrate-bound SMG1-8-9 kinase complex reveals molecular basis for phosphorylation specificity," ELIFE 9, e57127 (2020).
Virginia Busetto, Isabelle Barbosa, Jerome Basquin, Emelie Marquenet, Remi Hocq, Magali Hennion, Janio Antonio Paternina, Abdelkader Namane, Elena Conti, Olivier Bensaude, and Herve Le Hir, "Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes," Nucleic Acids Research (London) 48 (10), 5670-5683 (2020).
Achim Keidel, Elena Conti, and Sebastian Falk, "Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies", in Methods in Enzymology, 2062: Eukaryotic RNA Exosome: Methods and Protocols, (Humana Press Inc., 2020), Vol. 2062, pp. 491-513.
2019
Yair Gat, Jan Michael Schuller, Mahesh Lingaraju, Elisabeth Weyher, Fabien Bonneau, Mike Strauss, Peter J. Murray, and Elena Conti, "InsP(6) binding to PIKK kinases revealed by the cryo-EM structure of an SMG1-SMG8-SMG9 complex," Nature Structural and Molecular Biology 26 (12), 1089-1093 (2019).
Mahesh Lingaraju, Dennis Johnsen, Andreas Schlundt, Lukas M. Langer, Jerome Basquin, Michael Sattler, Torben Heick Jensen, Sebastian Falk, and Elena Conti, "The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs," Nature Communications 10, 3393 (2019).
Ingmar B. Schäfer, Masami Yamashita, Jan Michael Schuller, Steffen Schüssler, Peter Reichelt, Mike Strauss, and Elena Conti, "Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase," Cell 177 (6), 1619-1631.e21 (2019).
2018
Piotr Gerlach, Jan M. Schuller, Fabien Bonneau, Jerome Basquin, Peter Reichelt, Sebastian Falk, and Elena Conti, "Distinct and evolutionary conserved structural features of the human nuclear exosome complex," eLife 7, e38686 (2018).
Jan Michael Schuller, Sebastian Falk, Lisa Fromm, Ed Hurt, and Elena Conti, "Structure of the nuclear exosome captured on a maturing preribosome," Science 360 (6385), 219-222 (2018).
2017
Lisa Fromm, Sebastian Falk, Dirk Flemming, Jan Michael Schuller, Matthias Thoms, Elena Conti, and Ed Hurt, "Reconstitution of the complete pathway of ITS2 processing at the pre-ribosome," Nature Communications 8, 1787 (2017).
Sebastian Falk, Jan-Niklas Tants, Jerome Basquin, Matthias Thoms, Ed Hurt, Michael Sattler, and Elena Conti, "Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the preribosomal protein Nop53," RNA 23 (12), 1780-1787 (2017).
Sebastian Falk, Fabien Bonneau, Judith Ebert, Alexander Kögel, and Elena Conti, "Mpp6 Incorporation in the Nuclear Exosome Contributes to RNA Channeling through the Mtr4 Helicase," Cell Reports 20 (10), 2279-2286 (2017).
Bronislava Leonaite, Zhong Han, Jerome Basquin, Fabien Bonneau, Domenico Libri, Odil Porrua, and Elena Conti, "Sen1 has unique structural features grafted on the architecture of the Upf1-like helicase family," EMBO Journal 36 (11), 1590-1604 (2017).
Liang Li, Mahesh Lingaraju, Claire Basquin, Jerome Basquin, and Elena Conti, "Structure of a SMG8-SMG9 complex identifies a G-domain heterodimer in the NMD effector proteins," RNA 23 (7), 1028-1034 (2017).
2016
Christian Schmidt, Eva Kowalinski, Vivekanandan Shanmuganathan, Quentin Defenouillere, Katharina Braunger, Andre Heuer, Markus Pech, Abdelkader Namane, Otto Berninghausen, Micheline Fromont-Racine, Alain Jacquier, Elena Conti, Thomas Becker, and Roland Beckmann, "The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex," Science 354 (6318), 1431-1433 (2016).
Sebastian Falk, Ksenia Finogenova, Mireille Melko, Christian Benda, Soren Lykke-Andersen, Torben Heick Jensen, and Elena Conti, "Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors," Nature Communications 7, 13573 (2016).
Eva Kowalinski, Alexander Kögel, Judith Ebert, Peter Reichelt, Elisabeth Stegmann, Bianca Habermann, and Elena Conti, "Structure of a Cytoplasmic 11-Subunit RNA Exosome Complex," Molecular Cell 63 (1), 125-134 (2016).
Katharina Nakel, Fabien Bonneau, Claire Basquin, Bianca Habermann, Christian R. Eckmann, and Elena Conti, "Structural basis for the antagonistic roles of RNP-8 and GLD-3 in GLD-2 poly(A)-polymerase activity," RNA 22 (8), 1139-1145 (2016).
Lela Vukovic, Christophe Chipot, Debora L. Makino, Elena Conti, and Klaus Schulten, "Molecular Mechanism of Processive 3 ' to 5 ' RNA Translocation in the Active Subunit of the RNA Exosome Complex," JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138 (12), 4069-4078 (2016).
2015
J. Rajan Prabu, Marisa Müller, Andreas W. Thomae, Steffen Schüssler, Fabien Bonneau, Peter B. Becker, and Elena Conti, "Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling," MOLECULAR CELL 60 (3), 487-499 (2015).
Sevim Ozgur, Jerome Basquin, Anastasiia Kamenska, Witold Filipowicz, Nancy Standart, and Elena Conti, "Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex," CELL REPORTS 13 (4), 703-711 (2015).
Debora Lika Makino, Benjamin Schuch, Elisabeth Stegmann, Marc Baumgärtner, Claire Basquin, and Elena Conti, "RNA degradation paths in a 12-subunit nuclear exosome complex," NATURE 524 (7563), 54-58 (2015).
Katharina Nakel, Fabien Bonneau, Christian R. Eckmann, and Elena Conti, "Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3," PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112 (28), 8614-8619 (2015).
Kundan Sharma, Ajla Hrle, Katharina Kramer, Timo Sachsenberg, Raymond H.J. Staals, Lennart Randau, Anita Marchfelder, John van der Oost, Oliver Kohlbacher, Elena Conti, and Henning Urlaub, "Analysis of protein–RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry.," Methods: A Companion to Methods in Enzymology. 89 (Special Issue: Mass Spectrometry-Based Structural Biology), 138-148 (2015).
Eva Kowalinski, Anthony Schuller, Rachel Green, and Elena Conti, "Saccharomyces cerevisiae Ski7 Is a GTP-Binding Protein Adopting the Characteristic Conformation of Active Translational GTPases," STRUCTURE 23 (7), 1336-1343 (2015).
Varun Bhaskar, Jerome Basquin, and Elena Conti, "Architecture of the Ubiquitylation Module of the Yeast Ccr4-Not Complex," STRUCTURE 23 (5), 921-928 (2015).
Sevim Ozgur, Gretel Buchwald, Sebastian Falk, Sutapa Chakrabarti, Jesuraj Rajan Prabu, and Elena Conti, "The conformational plasticity of eukaryotic RNA-dependent ATPases.," The FEBS Journal 282 (5), 850-863 (2015).
2014
Benjamin Schuch, Monika Feigenbutz, Debora L. Makino, Sebastian Falk, Claire Basquin, Phil Mitchell, and Elena Conti, "The exosome-binding factors Rrp6 and Rrp47 form a composite surface for recruiting the Mtr4 helicase.," The EMBO journal 33 (23), 2829-2846 (2014).
Christian Benda, Judith Ebert, Richard A. Scheltema, Herbert B. Schiller, Marc Baumgärtner, Fabien Bonneau, Matthias Mann, and Elena Conti, "Structural Model of a CRISPR RNA-Silencing Complex Reveals the RNA-Target Cleavage Activity in Cmr4," MOLECULAR CELL 56 (1), 43-54 (2014).
Sebastian Falk, John R. Weir, Jendrik Hentschel, Peter Reichelt, Fabien Bonneau, and Elena Conti, "The Molecular Architecture of the TRAMP Complex Reveals the Organization and Interplay of Its Two Catalytic Activities," MOLECULAR CELL 55 (6), 856-867 (2014).
Ajla Hrle, L. K. Maier, K. Sharma, Judith Ebert, Claire Basquin, H. Urlaub, A. Marchfelder, and Elena Conti, "Structural analyses of the CRISPR protein Csc2 reveal the RNA-binding interface of the type I-D Cas7 family.," RNA Biology 11 (8), 1072-1082 (2014).
Michiel van de Waterbeemd, Philip Lössl, Violette Gautier, Fabio Marino, Masami Yamashita, Elena Conti, Arjen Scholten, and Albert J. R. Heck, "Simultaneous Assessment of Kinetic, Site-Specific, and Structural Aspects of Enzymatic Protein Phosphorylation," ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53 (36), 9660-9664 (2014).
Sutapa Chakrabarti, Fabien Bonneau, Steffen Schüssler, Elfriede Eppinger, and Elena Conti, "Phospho-dependent and phospho-independent interactions of the helicase UPF1 with the NMD factors SMG5-SMG7 and SMG6," NUCLEIC ACIDS RESEARCH 42 (14), 9447-9460 (2014).
Ingmar B. Schäfer, Michaela Rode, Fabien Bonneau, Steffen Schüssler, and Elena Conti, "The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase," NATURE STRUCTURAL & MOLECULAR BIOLOGY 21 (7), 591-598 (2014).
Hansruedi Mathys, Jerome Basquin, Sevim Ozgur, Mariusz Czarnocki-Cieciura, Fabien Bonneau, Aafke Aartse, Andrzej Dziembowski, Marcin Nowotny, Elena Conti, and Witold Filipowicz, "Structural and Biochemical Insights to the Role of the CCR4-NOT Complex and DDX6 ATPase in MicroRNA Repression," MOLECULAR CELL 54 (5), 751-765 (2014).
André Plagens, Vanessa Tripp, Michael Daume, Kundan Sharma, Andreas Klingl, Ajla Hrle, Elena Conti, Henning Urlaub, and Lennart Randau, "In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex.," Nucleic Acids Research (London) 42 (8), 5125-5138 (2014).
2013
Gretel Buchwald, Steffen Schüssler, Claire Basquin, Herve Le Hir, and Elena Conti, "Crystal structure of the human eIF4AIII-CWC22 complex shows how a DEAD-box protein is inhibited by a MIF4G domain," Proceedings of the National Academy of Sciences of the United States of America 110 (48), E4611-E4618 (2013).
Debora Lika Makino and Elena Conti, "Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement," ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 69, 2226-2235 (2013).
Humayun Sharif and Elena Conti, "Architecture of the Lsm1-7-Pat1 Complex: A Conserved Assembly in Eukaryotic mRNA Turnover," CELL REPORTS 5 (2), 283-291 (2013).
V. Bhaskar, V. Roudko, J. Basquin, K. Sharma, H. Urlaub, B. Seraphin, and E. Conti, "Structure and RNA-binding properties of the Not1-Not2Not5 module of the yeast Ccr4-Not complex.," Nature Structrural and Molecular Biology 12 (20), 1281-1288 (2013).
Ajla Hrle, Andreas A. H. Su, Judith Ebert, Christian Benda, Lennart Randau, and Elena Conti, "Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3," RNA BIOLOGY 10 (11), 1670-1678 (2013).
Debora L. Makino, Felix Halbach, and Elena Conti, "The RNA exosome and proteasome: common principles of degradation control," NATURE REVIEWS MOLECULAR CELL BIOLOGY 14 (10), 654-660 (2013).
Felix Halbach, Peter Reichelt, Michaela Rode, and Elena Conti, "The Yeast Ski Complex: Crystal Structure and RNA Channeling to the Exosome Complex," CELL 154 (4), 814-826 (2013).
Humayun Sharif, Sevim Ozgur, Kundan Sharma, Claire Basquin, Henning Urlaub, and Elena Conti, "Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions," NUCLEIC ACIDS RESEARCH 41 (17), 8377-8390 (2013).
Holger von Moeller, Rachel Lerner, Adele Ricciardi, Claire Basquin, William F. Marzluff, and Elena Conti, "Structural and biochemical studies of SLIP1-SLBP identify DBP5 and eIF3g as SLIP1-binding proteins," NUCLEIC ACIDS RESEARCH 41 (16), 7960-7971 (2013).
Debora L. Makino, Marc Baumgärtner, and Elena Conti, "Crystal structure of an RNA-bound 11-subunit eukaryotic exosome complex," NATURE 495 (7439), 70-75 (2013).
Thomas Holder, Claire Basquin, Judith Ebert, Nadine Randel, Didier Jollivet, Elena Conti, Gaspar Jekely, and Fulvia Bono, "Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability," BIOLOGY DIRECT 8, 2 (2013).
2012
Esben Lorentzen and Elena Conti, "Crystal Structure of a 9-Subunit Archaeal Exosome in Pre-Catalytic States of the Phosphorolytic Reaction," ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL , 721869 (2012).
Jerome Basquin, Vladimir V. Roudko, Michaela Rode, Claire Basquin, Bertrand Seraphin, and Elena Conti, "Architecture of the Nuclease Module of the Yeast Ccr4-Not Complex: the Not1-Caf1-Ccr4 Interaction," Molecular Cell 48 (2), 207-218 (2012).
R. Melero, G. Buchwald, R. Castano, M. Raabe, D. Gil, M. Lazaro, H. Urlaub, E. Conti, and O. Llorca, "The cryo-EM structure of the UPF-EJC complex shows UPF1 poised toward the RNA 3 ' end," Nature Structural & Molecular Biology 19 (5), 498-595 (2012).
A. A. Jeyaprakash, A. Santamaria, U. Jayachandran, Y. W. Chan, C. Benda, E. A. Nigg, and E. Conti, "Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface," Molecular Cell 46 (3), 274-286 (2012).
A. G. Murachelli, J. Ebert, C. Basquin, H. Le Hir, and E. Conti, "The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex," Nature Structural & Molecular Biology 19 (4), 378-386 (2012).
F. Halbach, M. Rode, and E. Conti, "The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome," RNA-A Publication of the RNA Society 18 [2012] (1), 124-134 (2011).
2011
A. A. Jeyaprakash, C. Basquin, U. Jayachandran, and E. Conti, "Structural Basis for the Recognition of Phosphorylated Histone H3 by the Survivin Subunit of the Chromosomal Passenger Complex," Structure 19 (11), 1625-1634 (2011).
S. Chakrabarti, U. Jayachandran, F. Bonneau, F. Fiorini, C. Basquin, S. Domcke, H. Le Hir, and E. Conti, "Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2," Molecular Cell 41 (6), 693-703 (2011).
2010
H. Malet, M. Topf, D. K. Clare, J. Ebert, F. Bonneau, J. Basquin, K. Drazkowska, R. Tomecki, A. Dziembowski, E. Conti, H. R. Saibil, and E. Lorentzen, "RNA channelling by the eukaryotic exosome," EMBO Reports 11 (12), 936-942 (2010).
I. Kashima, S. Jonas, U. Jayachandran, G. Buchwald, E. Conti, A. N. Lupas, and E. Izaurralde, "SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay," Genes & Development 24 (21), 2440-2450 (2010).
K. Nakel, S. A. Hartung, F. Bonneau, C. R. Eckmann, and E. Conti, "Four KH domains of the C. elegans Bicaudal-C ortholog GLD-3 form a globular structural platform," RNA-A Publication of the RNA Society 16 (11), 2058-2067 (2010).
J. R. Weir, F. Bonneau, J. Hentschel, and E. Conti, "Structural analysis reveals the characteristic features of Mtr4, a DExH helicase involved in nuclear RNA processing and surveillance," Proceedings of the National Academy of Sciences of the United States of America 107 (27), 12139-12144 (2010).
G. Buchwald, J. Ebert, C. Basquin, J. Sauliere, U. Jayachandran, F. Bono, H. Le Hir, and E. Conti, "Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.," Proceedings of the National Academy of Sciences of the United States of America 107 (22), 10050-10055 (2010).
A. G. Cook and E. Conti, "Nuclear export complexes in the frame," Current Opinion in Structural Biology 20 (2), 247-252 (2010).
F. Bono, A. G. Cook, M. Grünwald, J. Ebert, and E. Conti, "Nuclear Import Mechanism of the EJC Component Mago-Y14 Revealed by Structural Studies of Importin 13," Molecular Cell 37 (2), 211-222 (2010).
2009
F. Bonneau, J. Basquin, J. Ebert, E. Lorentzen, and E. Conti, "The Yeast Exosome Functions as a Macromolecular Cage to Channel RNA Substrates for Degradation," Cell 139 (3), 547-559 (2009).
A. G. Cook, N. Fukuhara, M. Jinek, and E. Conti, "Structures of the tRNA export factor in the nuclear and cytosolic states," Nature 461 (7260), 60-65 (2009).
T. N. Gaitanos, A. Santamaria, A. A. Jeyaprakash, B. Wang, E. Conti, and E. A. Nigg, "Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3," EMBO Journal 28 (10), 1442-1452 (2009).
H. von Moeller, C. Basquin, and E. Conti, "The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner," Nature Structural & Molecular Biology 16 (3), 247-254 (2009).
2008
E. Lorentzen and E. Conti, "Expression, reconstitution, and structure of an archaeal RNA degrading exosome", in RNA Turnover in Prokaryotes, Archaea and Organelles, (Elsevier Academic Press Inc., San Diego, 2008), pp. 417-435.
E. Lorentzen, J. Basquin, and E. Conti, "Structural organization of the RNA-degrading exosome," Current Opinion in Structural Biology 18 (6), 709-713 (2008).
M. Jinek, A. Eulalio, A. Lingel, S. Helms, E. Conti, and E. Izaurralde, "The C-terminal region of Ge-1 presents conserved structural features required for P-body localization," RNA-A Publication of the RNA Society 14 (10), 1991-1998 (2008).
E. Lorentzen, J. Basquin, R. Tomecki, A. Dziembowski, and E. Conti, "Structure of the active subunit of the yeast exosome core, Rrp44: Diverse modes of substrate recruitment in the RNase II nuclease family," MOLECULAR CELL 29 (6), 717-728 (2008).
2007
A. A. Jeyaprakash, U. R. Klein, D. Lindner, J. Ebert, E. A. Nigg, and E. Conti, "Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together," Cell 131 (2), 271-285 (2007).
A. Cook, F. Bono, M. Jinek, and E. Conti, "Structural biology of nucleocytoplasmic transport," Annual Review of Biochemistry 76, 647-671 (2007).
E. Lorentzen, A. Dziembowski, D. Lindner, B. Seraphin, and E. Conti, "RNA channelling by the archaeal exosome," EMBO Reports 8, 470-476 (2007).
A. Dziembowski, E. Lorentzen, E. Conti, and B. Seraphin, "A single subunit, Dis3, is essentially responsible for yeast exosome core activity," Nature Structural & Molecular Biology 14 (1), 15-22 (2007).
A. Oddone, E. Lorentzen, J. Basquin, A. Gasch, V. Rybin, E. Conti, and M. Sattler, "Structural and biochemical characterization of the yeast exosome component Rrp40," EMBO Reports 8 (1), 63-69 (2007).
2006
F. Glavan, I. Behm-Ansmant, E. Izaurralde, and E. Conti, "Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex," EMBO Journal 25 (21), 5117-5125 (2006).
M. Jinek, Y. W. Chen, H. Clausen, S. M. Cohen, and E. Conti, "Structural insights into the Notch-modifying glycosyltransferase Fringe," Nature Structural & Molecular Biology 13 (10), 945-946 (2006).
F. Bono, J. Ebert, E. Lorentzen, and E. Conti, "The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA," Cell 126 (4), 713-725 (2006).
M. Jinek and E. Conti, "Eukaryotic expression, purification, crystallization and preliminary X-ray analysis of murine Manic Fringe," Acta Crystallographica Section F-Structural Biology and Crystallization Communications 62, 774-777 (2006).
E. Lorentzen and E. Conti, "The exosome and the proteasome: Nano-compartments for degradation," Cell 125 (4), 651-654 (2006).
Chemical shift backbone assignments of TAP-N, the 31 kDa cargo-binding region of the protein TAP.
Journal of Biomolecular NMR, 01 Jan 2006, 36 Suppl 1:23
Karyopherin flexibility in nucleocytoplasmic transport.
Current Opinion in Structural Biology, 29 Mar 2006, 16(2):237-244
2005
Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core.
Lorentzen E, Conti E.
Mol Cell. 2005 Nov 11;20(3):473-81.
Pubmed
The archaeal exosome core is a hexameric ring structure with three catalytic subunits.
Lorentzen E, Walter P, Fribourg S, Evguenieva-Hackenberg E, Klug G, Conti E.
Nat Struct Mol Biol. 2005 Jul;12(7):575-81. Pubmed
Nonsense-mediated mRNA decay: molecular insights and mechanistic variations across species.
Conti E, Izaurralde E.
Curr Opin Cell Biol. 2005 Jun;17(3):316-25. Review. Pubmed
The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding.
Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E.
Mol Cell. 2005 Apr 29;18(3):355-67. Pubmed
SMG7 is a 14-3-3-like adaptor in the nonsense-mediated mRNA decay pathway.
Fukuhara N, Ebert J, Unterholzner L, Lindner D, Izaurralde E, Conti E.
Mol Cell. 2005 Feb 18;17(4):537-47. Pubmed
2004
The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin alpha.
Jinek M, Rehwinkel J, Lazarus BD, Izaurralde E, Hanover JA, Conti E.
Nat Struct Mol Biol. 2004 Oct;11(10):1001-7. Pubmed
Determinants for Aurora-A activation and Aurora-B discrimination by TPX2.
Bayliss R, Sardon T, Ebert J, Lindner D, Vernos I, Conti E.
Cell Cycle. 2004 Apr;3(4):404-7. Pubmed
Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex.
Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E.
EMBO Rep. 2004 Mar;5(3):304-10. Pubmed
Conformational variability of nucleo-cytoplasmic transport factors.
Fukuhara N, Fernandez E, Ebert J, Conti E, Svergun D.
J Biol Chem. 2004 Jan 16;279(3):2176-81. Pubmed
2003
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
Bayliss R, Sardon T, Vernos I, Conti E.
Mol Cell. 2003 Oct;12(4):851-62. Pubmed
Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15.
Fribourg S, Conti E.
EMBO Rep. 2003 Jul;4(7):699-703. Pubmed
A novel mode of RBD-protein recognition in the Y14-Mago complex.
Fribourg S, Gatfield D, Izaurralde E, Conti E.
Nat Struct Biol. 2003 Jun;10(6):433-9. Pubmed
2002
Structures of importins.
Conti E.
Results Probl Cell Differ. 2002;35:93-113. Review. Pubmed
2001
Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor.
Fribourg S, Braun IC, Izaurralde E, Conti E.
Mol Cell. 2001 Sep;8(3):645-56. Pubmed
Nucleocytoplasmic transport enters the atomic age.
Conti E, Izaurralde E.
Curr Opin Cell Biol. 2001 Jun;13(3):310-9. Review. Pubmed
Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.
Braun IC, Herold A, Rode M, Conti E, Izaurralde E.
J Biol Chem. 2001 Jun 8;276(23):20536-43. Pubmed
2000
The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain.
Liker E, Fernandez E, Izaurralde E, Conti E.
EMBO J. 2000 Nov 1;19(21):5587-98. Pubmed
Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha.
Conti E, Kuriyan J.
Structure. 2000 Mar 15;8(3):329-38. Pubmed
1998
Structural basis for the inhibition of firefly luciferase by a general anesthetic.
Franks NP, Jenkins A, Conti E, Lieb WR, Brick P.
Biophys J. 1998 Nov;75(5):2205-11. Pubmed
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha.
Conti E, Uy M, Leighton L, Blobel G, Kuriyan J.
Cell. 1998 Jul 24;94(2):193-204. Pubmed
X-ray and spectrophotometric studies of the binding of proflavin to the S1 specificity pocket of human alpha-thrombin.
Conti E, Rivetti C, Wonacott A, Brick P.
FEBS Lett. 1998 Mar 27;425(2):229-33. Pubmed