Dr. Adam Mamot

PostDoc

I graduated from the University of Warsaw with a bachelor's degree in chemistry, where I was awarded the best bachelor's thesis for my research on click chemistry-based methods for mRNA detection. I then completed a PhD focusing on RNA modification techniques, which led to advances in mRNA molecular probes and the development of chemically modified circular RNA. I was awarded the START Prize from the Foundation for Polish Science for my contributions. In 2023, I received a fellowship from the Alexander von Humboldt Foundation to join Petra Schwille's lab at the Max Planck Institute of Biochemistry, where I am designing and studying the properties of minimal gene expression machinery in the context of lipid membranes. Recently, I was awarded the Marie Skłodowska-Curie Postdoctoral Fellowship to develop self-assembling systems for therapeutic mRNA production and delivery.

Research Interests

mRNA therapeutics, RNA transcription and modification, molecular self-organization and compartmentalization, synthetic biology, gene delivery systems.

Publications

Adam Mamot, Małgorzata Wąsińska-Kałwa, Karol Czubak, Katarzyna Frankowska, Tomasz Śpiewla, Marcin Warmiński, Dominika Nowis, Jakub Gołąb, Joanna Kowalska, Jacek Jemielity “Chemical circularization of in vitro transcribed RNA opens new avenues for circular mRNA design” bioRxiv 2024 DOI: 10.1101/2024.10.10.617555

Grzegorz Bubak, Antoni Lis, Krzysztof Kolankowski, Adam K. Kowalski, Adam Mamot, Olga Perzanowska, Krzysztof Bielec, Joanna Kowalska, Krzysztof Burdzy, Jacek Jemielity, Robert Holyst “smICA: an open source repository for mapping the concentration of fluorescently labeled molecules in living cells on the basis of confocal imaging combined with time-correlated single-photon counting” arXiv 2024, DOI: 10.48550/arXiv.2410.00532

Marcin Warminski, Edyta Trepkowska, Miroslaw Smietanski, Pawel J. Sikorski, Marek R. Baranowski, Marcelina Bednarczyk, Hanna Kedzierska, Bartosz Majewski, Adam Mamot, Diana Papiernik, Agnieszka Popielec, Remigiusz A. Serwa, Brittany A. Shimanski, Piotr Sklepkiewicz, Marta Sklucka, Olga Sokolowska, Tomasz Spiewla, Diana Toczydlowska-Socha, Zofia Warminska, Karol Wolosewicz, Joanna Zuberek, Jeffrey S. Mugridge, Dominika Nowis, Jakub Golab, Jacek Jemielity, Joanna Kowalska “Trinucleotide mRNA Cap Analogue N6-Benzylated at the Site of Posttranscriptional m6Am Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo” Journal of the American Chemical Society 2024, DOI: 10.1021/jacs.3c12629

Rafal Tomecki, Karolina Drazkowska, Rafal Madaj, Adam Mamot, Stanislaw Dunin‐Horkawicz, Pawel J Sikorski “Expanding the Available RNA Labeling Toolbox With CutA Nucleotidyltransferase for Efficient Transcript Labeling with Purine and Pyrimidine Nucleotide Analogs” ChemBioChem 2024 DOI: 10.1002/cbic.202400202

Marcin Warmiński, Adam Mamot, Anais Depaïx, Joanna Kowalska, Jacek Jemielity „Chemical Modifications of mRNA Ends for Therapeutic Applications” Accounts of Chemical Research 2023, DOI: 10.1021/acs.accounts.3c00442

Adam Mamot, Paweł J. Sikorski, Aleksandra Siekierska, Peter de Witte, Joanna Kowalska, Jacek Jemielity, „Ethylenediamine derivatives efficiently react with oxidized RNA 3’ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation” Nucleic Acids Research2022, DOI: 10.1093/nar/gkab867

Renata Kasprzyk, Mateusz Fido, Adam Mamot, Przemysław Wanat, Mirosław Smietański, Michał Kopciał, Victoria Cowling, Joanna Kowalska, Jacek Jemielity, “Direct High‐Throughput Screening Assay for mRNA Cap Guanine‐N7 Methyltransferase Activity” Chemistry - A European Journal 2020, 26, 11266

Adam Mamot, Paweł J. Sikorski, Marcin Warmiński, Joanna Kowalska, Jacek Jemielity, “Azido Functionalized 5’ Cap Analogues for the Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells” Angewandte Chemie International Edition 2017, 55, 15628.

Patent applications

Adam Mamot, Paweł Sikorski, Joanna Kowalska, Jacek Jemielity “N-(2-aminoetylo)morfolinowe analogi RNA, sposób ich otrzymywania i zastosowanie” (N-(2-aminoethyl)morpholine RNA analogs, method for the preparation and use thereof). International patent application (PCT): WO2022191725A1, US20240182512A1, EP4305045A1, CA3211579A1, JP2024510598A, AU2022234261A1, KR20230157386A