Measuring the tRNA world by mim-tRNAseq

Transfer RNAs (tRNAs) specifically deliver amino acids to ribosomes during the translation of messenger RNA (mRNA) into proteins. The abundance of tRNAs therefore has a profound impact on cells. However, determining the amount of each tRNA species in cells was limited because of technical challenges. We overcame these limitations thanks to mim-tRNAseq, a method that can be used to quantify tRNAs in any organism and will help improve our understanding of tRNA regulation in health and disease.